Hongxia Hao
China University of Political Science and Law, China
Title: TNT detection based on aptamers and gold nanoparticles-enhanced surface plasmon resonance biosensor
Biography
Biography: Hongxia Hao
Abstract
At present, there are varied detection methods for TNT, which can be mainly divided into the traditional technology and new type of micro trace detection technology. The common ways of bulk explosive quick detecting technology are X-ray detection technology, THZ spectroscopy, ion mobility spectrometry, portable Raman spectroscopy and so on, which generally have flaws like high costs, weak sensitivity, defeating in recognize the mixture and complex, a false positive or false negative, bulky, expensive. Herein, a novel quick testing method was developed using DNA aptamer and surface plasma resonance technology (SPR). The gold nanoparticles (AuNPs) are used to enhance the surface plasma resonance signal (SESS), so that TNT can be detected directly and rapidly by highly sensitive SPR sensor. TNT aptamer screened by System ligand evolution technology (SELEX) was added to modify on AuNPs. Based on adapter competition between TNT and TNP–gly, microtrace of TNT in the solution can be detected with LOD of 10-9M. The linear ship of TNT concentration is 10-9-10-6M (r2=0.989). Herein it is the first time that aptamer was used with SPR sensor chip modified by nanoparticles to detect TNT. This method help forensic scientist discover trace explosive with portable instrument.